A leading CRO specializing in cell culture is dedicated to providing high-quality iPSC scale-up culture services to research institutions globally.
iPSCs, with their significant potential for disease modeling and drug screening, require rigorous maintenance of pluripotency and viability to fully realize their application value. "Therefore, we place a strong emphasis on iPSC pluripotency characterization services," explains a field expert.
Accurately validating and detecting iPSC pluripotency markers is essential for effective cell proliferation and differentiation. To address this, a comprehensive range of solutions is available to monitor cell pluripotency and genomic stability during large-scale cultures.
Quantitative polymerase chain reaction (qPCR) is a state-of-the-art technique used to monitor the amplification of target DNA in real-time. This technology allows for precise and quantitative measurement of pluripotency markers in iPSCs, including OCT4, SOX2, and NANOG.
"Our advanced qPCR services for iPSCs encompass RNA extraction, cDNA synthesis, primer design and validation, qPCR analysis, and detailed data interpretation," the expert added.
In addition to qPCR, in vitro assessment of iPSC pluripotency is crucial. Reprogramming adult somatic cells to an embryonic-like state for iPSC differentiation has been shown to be effective. "We offer solutions for generating embryoid bodies from iPSCs, which provides a reliable method for in vitro pluripotency assessment."
For large-scale iPSC cultures, it is important to manage the complement system, which can impact cell growth and differentiation through various pathways. Specifically, anti-factor H autoantibodies can inhibit factor H, leading to complement system overactivation, inflammation, and cell damage.
Such overactivation can affect iPSC survival and pluripotency maintenance. To address this, comprehensive anti-factor H autoantibody testing services are available to ensure that iPSCs remain unaffected by complement system dysregulation during culture.
"Advanced culture systems, including suspension culture, microcarrier culture, and bioreactors, are employed to optimize iPSC expansion efficiency. Additionally, adjusting culture conditions (e.g., oxygen levels, pH, stirring speed) is essential for maximizing cell yield and quality," the expert noted.
With the growing need for large quantities of human iPSCs for drug screening, we provide various iPSC culture methods tailored to meet researchers' specific experimental needs.
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The company is committed to supporting disease modeling and drug screening for research institutions worldwide, continually advancing technological innovation and process optimization to ensure the highest quality and pluripotency of every batch of iPSCs.
